Retinol, alpha-tocopherol, lycopene, and alpha- and beta-carotene simultaneously determined in plasma by isocratic liquid chromatography.
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منابع مشابه
Determination of retinol, alpha-tocopherol, and beta-carotene in serum by liquid chromatography with absorbance and electrochemical detection.
We describe a method for the determination of retinol, alpha-tocopherol, and beta-carotene in serum, using a liquid-chromatographic separation with wavelength-programmed ultraviolet/visible absorbance and amperometric electrochemical detection with a glassy carbon electrode. After protein denaturation and addition of an internal standard, tocol, 250-microL samples are twice extracted with hexan...
متن کاملSimultaneous determination of retinol and alpha-tocopherol in serum or plasma by liquid chromatography.
Submitters: George L. Catignani, Department of Food Science, North Carolina State University, Raleigh, NC 27650 John G. Bieri, Nutritional Biochemistry Section, Laboratory of Nutrition and Endocrinology, National Institute of Arthritis, Metabolism, and Digestive Diseases, Bethesda, MD 20014 Evaluators: William J. Driskell and Mark M. Bashor, NutriLional Biochemistry Branch, Clinical Chemistry D...
متن کاملDietary carotenoids, serum beta-carotene, and retinol and risk of lung cancer in the alpha-tocopherol, beta-carotene cohort study.
Findings from several beta-carotene supplementation trials were unexpected and conflicted with most observational studies. Carotenoids other than beta-carotene are found in a variety of fruits and vegetables and may play a role in this important malignancy, but previous findings regarding the five major carotenoids are inconsistent. The authors analyzed the associations between dietary beta-car...
متن کاملDetermination of vitamins A (retinol) and E (alpha-tocopherol) in foods by liquid chromatography: collaborative study.
A collaborative study was conducted for the determination of vitamins A and E. Existing AOAC liquid chromatographic (LC) methods are suited for specific vitamins A and E analytical applications. This method differs from existing methods in that it can be used to assay samples in all 9 sectors of the food matrix. Standards and test samples are saponified in basic ethanol-water solution, neutrali...
متن کاملDietary Carotenoids, Serum β-Carotene, and Retinol and Risk of Lung Cancer in the Alpha-Tocopherol, Beta-Carotene Cohort Study
Findings from several β-carotene supplementation trials were unexpected and conflicted with most observational studies. Carotenoids other than β-carotene are found in a variety of fruits and vegetables and may play a role in this important malignancy, but previous findings regarding the five major carotenoids are inconsistent. The authors analyzed the associations between dietary β-carotene, β-...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1986
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/32.5.874